Generator

Part:BBa_I716340:Design

Designed by: nhu nguyen   Group: iGEM07_Berkeley_UC   (2007-08-14)


rbs-thpD-TT


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 1059
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal NgoMIV site found at 666
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 483
    Illegal SapI site found at 722


Design Notes

Digest I716338 (EcoRI/BamHI, 2057bp, 923bp, S) Sub into Sub into pBca9145-Bca1092 (EcoRI/BglII 2198bp, 9bp, L) Product is I716340


Source


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

References