Part:BBa_I716316:Design

H-NOX

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

Source

PCR HNX-F and HNX-R on H-Nox (567bp, BglII/XhoI) Sub into pBca1144 ( BglII/XhoI, 2054+910 L) Product is BBA=a_1716316 ---------------------------------------------------------- HNX-F Forward primer anneals to BglII ctagtAGATCTATGAAGGGGACAATCGTCGG

HNX-R Reverse primer anneals to XhoI cgtgaCTCGAGgaatgGGATCCTTAATTTTTCTTATACTCA