Composite

Part:BBa_I716306:Design

Designed by: vincent parker   Group: iGEM07_Berkeley_UC   (2007-07-24)

T7-RBS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 74
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 74
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal BamHI site found at 59
    Illegal XhoI site found at 68
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 74
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 74
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1155


Design Notes

Biobrickificreation of T7 Promoters I716105

PCR ca1090F and dt008-PT7-wt (62 bp, EcoRI/BamHI, Product = A) Sub into pBca9145-Bca1144 (EcoRI/BamHI, 2063+910, L) Product A is pBca9145-I716105-PT7-wt

dt008-PT7-wt T7 Promoter (Wild Type) GtttGGATCCaccTCTCCCTATAGTGAGTCGTATTAtacAGATCTcatGAATTCcagaa

Sub into pJD507_pSB1A2C (EcoRI, BglII 2071bp, 9bp L) Produst is I716306



Source


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

References