Coding

Part:BBa_I716253:Design

Designed by: Vaibhavi Umesh   Group: iGEM07_Berkeley_UC   (2007-06-06)

HPI, katG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1618
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1290
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 772


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

Construction of a Biobrick 2.0 basic part in plasmid pBca9145-Bca1144 (Clone # 5) for the HPI, katG open reading frame from Salmonella typhimurium LT2 

PCR VU03F.katG /VU06R.katG on HPI, katG		(1316 bp, gp = A)

PCR VU05F.katG/VU04R.katG on HPI, katG		(924 bp, gp = B)

---------------------------------------------------

PCR VU03F/VU04R on A+B				(2212 bp, BglII/XhoI)

Digest pBca9145-Bca1144 (Clone # 5)		(BglII/XhoI, 2063+910, L)

Product is pBca9145-VU2 

---------------------------------------------------
VU03F.katG (External oligo) 	ccaaaAGATCTatgagcacgaccgacgataccc
VU04R.katG (External oligo) 	gctagCTCGAGttaGGATCCttattgcagatcgaaacggtcc
VU05F.katG (Internal oligo) 		gccgaaagaagaCctgatctggcag
VU06R.katG (Internal oligo) 		ctgccagatcagGtcttctttcggc


Source

Salmonella typhimurium LT2

References