Composite

Part:BBa_I716219:Design

Designed by: Samantha Liang   Group: iGEM07_Berkeley_UC   (2007-06-26)


[rbs][ATG][lox][dblterm][lox][BamHI]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 913
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 913
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 898
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 913
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2000


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

Digest pBca9145-I716215 (BamHI/AlwnI/BglI, 1166+596+553bp, Large) Digest pBca9145-I716210 (BglII/BglI, 1794+923bp, Large) Product is pBca9145-I716219.


Source

Digest pBca9145-I716215 (BamHI/AlwnI/BglI, 1166+596+553bp, Large) Digest pBca9145-I716210 (BglII/BglI, 1794+923bp, Large) Product is pBca9145-I716219.

References