Other

Part:BBa_I716160:Design

Designed by: Kristin Doan   Group: iGEM07_Berkeley_UC   (2007-08-08)


[Library RBS]hemA from CFT073


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites. 

Composite part of RBS (pBca1101-I716051-kan cassette) + hemA from CFT073
Digest pBca1101-I716051		        (EcoR1/BamH1, 2904+1065, S)
Sub into pBca9145-I71652		(EcoR1/Bg1ll, 3326+9, L)
Product is pBca9145-I716160



Source

Composite part

References