Regulatory

Part:BBa_I714930:Design

Designed by: Chunbo Lou   Group: iGEM07_Peking   (2007-10-22)


RecA_D_consenLexO_lacO1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

a NOR gate

wild type recA promoter, with two lexA binding sites and two lacI binding site. The lexA binding site is the consensus sequence of lexA operator, the lacI binding site is lacO1. One of the lexA binding sites is at the same location as wild type recA promoter and the other one is 30bp upstream. The two lacO1 sites locate at the same positions as in wild type lac promoter.

Source

synthetic

References