Regulatory

Part:BBa_I714929:Design

Designed by: Chunbo Lou   Group: iGEM07_Peking   (2007-10-22)


RecA_S_WTlexO_DLacO3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NOR gate

wild type recA promoter, with single lexA binding sites and two lacI binding site. The lexA binding site is wild type operator of recA promoter, the lacI binding site is lacO3. The lexA binding sites is at the same location as wild type recA promoter and the two lacO3 sites locate at the same positions as in wild type lac promoter.

Source

synthetic

References