Regulatory
Part:BBa_I714925:Design
Designed by: Chunbo Lou Group: iGEM07_Peking (2007-10-22)
RecA_DlexO_DLacO2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
wild type recA promoter, with two lexA binding sites and two lacI binding site. The lexA binding site is operator of recA promoter, the lacI binding site is lacO2. One of the lexA binding sites is at the same location as wild type recA promoter and the other one is 30bp upstream the wild type one. The two lacO2 sites locate at the same positions as in wild type lac promoter.
Source
synthetic