Coding
Part:BBa_I714002:Design
Designed by: Yang Yifan Group: iGEM07_Peking (2007-05-30)
traI of F plasmid
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 127
Illegal PstI site found at 2203
Illegal PstI site found at 2684
Illegal PstI site found at 3154
Illegal PstI site found at 3307
Illegal PstI site found at 3925
Illegal PstI site found at 4720 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 127
Illegal PstI site found at 2203
Illegal PstI site found at 2684
Illegal PstI site found at 3154
Illegal PstI site found at 3307
Illegal PstI site found at 3925
Illegal PstI site found at 4720 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 127
Illegal PstI site found at 2203
Illegal PstI site found at 2684
Illegal PstI site found at 3154
Illegal PstI site found at 3307
Illegal PstI site found at 3925
Illegal PstI site found at 4720 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 127
Illegal PstI site found at 2203
Illegal PstI site found at 2684
Illegal PstI site found at 3154
Illegal PstI site found at 3307
Illegal PstI site found at 3925
Illegal PstI site found at 4720
Illegal NgoMIV site found at 100
Illegal NgoMIV site found at 2586
Illegal NgoMIV site found at 3106
Illegal NgoMIV site found at 4279
Illegal NgoMIV site found at 4587
Illegal AgeI site found at 406
Illegal AgeI site found at 687
Illegal AgeI site found at 1621
Illegal AgeI site found at 1905
Illegal AgeI site found at 2695
Illegal AgeI site found at 4021
Illegal AgeI site found at 4858 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We do not have the actual DNA yet, Trying to PCR it from the F plasmid
Source
Sequence information from NCBI nucleotide database ACCESSION NC_002483 REGION: 92673..97943