Plasmid

Part:BBa_I713024:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pEG-FLP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1050
    Illegal XbaI site found at 1630
    Illegal SpeI site found at 258
    Illegal SpeI site found at 1368
    Illegal PstI site found at 1590
    Illegal PstI site found at 2780
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1050
    Illegal SpeI site found at 258
    Illegal SpeI site found at 1368
    Illegal PstI site found at 1590
    Illegal PstI site found at 2780
    Illegal NotI site found at 1611
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1050
    Illegal BglII site found at 2567
    Illegal BamHI site found at 252
    Illegal BamHI site found at 295
    Illegal XhoI site found at 1573
    Illegal XhoI site found at 1618
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1050
    Illegal XbaI site found at 1630
    Illegal SpeI site found at 258
    Illegal SpeI site found at 1368
    Illegal PstI site found at 1590
    Illegal PstI site found at 2780
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1050
    Illegal XbaI site found at 1630
    Illegal SpeI site found at 258
    Illegal SpeI site found at 1368
    Illegal PstI site found at 1590
    Illegal PstI site found at 2780
    Illegal NgoMIV site found at 1956
    Illegal NgoMIV site found at 3231
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4130
    Illegal SapI.rc site found at 3080
    Illegal SapI.rc site found at 3290


Design Notes

BamHI-FLP- XhoI


Source

pCP20

References