Plasmid

Part:BBa_I713008:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


AAV110


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2706
    Illegal XbaI site found at 721
    Illegal PstI site found at 1873
    Illegal PstI site found at 2636
    Illegal PstI site found at 3458
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2706
    Illegal PstI site found at 1873
    Illegal PstI site found at 2636
    Illegal PstI site found at 3458
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2706
    Illegal BglII site found at 655
    Illegal BamHI site found at 1887
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2706
    Illegal XbaI site found at 721
    Illegal PstI site found at 1873
    Illegal PstI site found at 2636
    Illegal PstI site found at 3458
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2706
    Illegal XbaI site found at 721
    Illegal PstI site found at 1873
    Illegal PstI site found at 2636
    Illegal PstI site found at 3458
    Illegal NgoMIV site found at 5
    Illegal NgoMIV site found at 623
    Illegal NgoMIV site found at 5784
    Illegal NgoMIV site found at 6138
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3632
    Illegal BsaI.rc site found at 2561
    Illegal SapI.rc site found at 4714


Design Notes

First insert LacI with NcoI and BamHI.

Then insert EGFP with BamHI and HindIII.


Source

BBa_I713036

References