Translational_Unit

Part:BBa_I712069:Design

Designed by: Sasa Jereb   Group: iGEM07_Ljubljana   (2007-10-22)


RBS-T7 RNA polymerase with NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2654
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

DNA was isolated from BL21 and PCR was performed to attach RBS and NLS.


Source

Genomic DNA of BL21 (E.coli strain).

References