Composite

Part:BBa_I712047:Design

Designed by: Katja Kolar   Group: iGEM07_Ljubljana   (2007-10-23)


CMVp - HA - CD4 - HIV protease cleavage site - T7RNAP - NLS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1307
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4783
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1920
    Illegal NgoMIV site found at 1969
    Illegal AgeI site found at 2050
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1754
    Illegal SapI.rc site found at 842


Design Notes

The construct was cloned into pSB1AK3 vector with terminator.

Source

For more information please check basic parts.

References