Part:BBa_I712004:Experience

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Please enter how you used this part and how it worked out.

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We validated this part by insert it and eGFP together into the vertor. The map of the plasmid is as followed(Fig.1). Then we transfected the plasmid to 293T cells, and the expression of eGFP protein was observed by fluorescence microscope(Fig.2) and quantified by Image J(Fig.3). You can also see the characterization of this part in our composite part BBa_K2942705, BBa_K2942706, BBa_K2942707, BBa_K2942708.

Fig.1 The plasmid we used for transfection.

Fig.2 Transfection cells observed under a fluorescence microscope.

Fig.3 Fluorescent quantitation by Image J(P<0.001).

UNIQ833ea4e11c9856d1-partinfo-00000000-QINU UNIQ833ea4e11c9856d1-partinfo-00000001-QINU