Composite

Part:BBa_I52000

Designed by: Reshma Shetty   Group:   (2006-03-13)

ccdB and minimal pUC19 derived high copy origin

This part is designed to be located in the multiple cloning site of low copy BioBrick vectors. BBa_P1011 provides selection against non-insert containing clones and BBa_I50020 enables the base vector to be propagated at high copy for ease of purification

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 443
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None