Part:BBa_I50041:Design

pSC101 origin of replication, reverse

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Two mutations were introduced in order to remove two MfeI sites. These two mutations occur within an inverted repeat and one also occurs within a ribosome binding site. It is unclear what impact these mutations will have on the replication origin.