DNA
F plasmid

Part:BBa_I50000:Design

Designed by: Reshma Shetty   Group:   (2005-11-16)


F plasmid backbone with BioBrick sites removed.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 133
    Illegal BglII site found at 2200
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 690
    Illegal NgoMIV site found at 4606
    Illegal AgeI site found at 814
    Illegal AgeI site found at 2044
    Illegal AgeI site found at 2937
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3791
    Illegal SapI site found at 1583
    Illegal SapI site found at 2793


Design Notes

Source

References

  • J. Wild, Z. Hradecna, and W. Szybalski. Conditionally amplifiable BACs: switching from single-copy to high-copy vectors and genomic clones. Genome Res, 12(1088-9051):1434,44, 2002. (http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12213781&query_hl=4)
  • pSMART VC vector from Lucigen http://www.lucigen.com/products/Cloning_Systems/CopyRight.html. GenBank accession number AY643800