Part:BBa_I20284:Design

Bacteriophage Bxb1 Integrase-Freiburg standard

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 198
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 472
  Illegal XhoI site found at 559
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 4
  Illegal AgeI site found at 1507
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1306
  

Design Notes

Sequences cleaned up for restriction sites according to Freiburg standard (25)

Source

Source: http://www.uniprot.org/uniprot/Q9B086

References

[1] Genome organization and characterization of mycobacteriophage Bxb1., mediavilla et al., Mol microbiol, 2000. PMID 1123671.

[2] Mycobacteriophage Bxb1 integrates into the Mycobacterium smegmatis groEL1 gene. Kim AI, Ghosh P, Aaron MA, Bibb LA, Jain S, Hatfull GF. Mol Microbiol. 2003

[3] The orientation of mycobacteriophage Bxb1 integration is solely dependent on the central dinucleotide of attP and attB. Ghosh P, Kim AI, Hatfull GF. Mol Cell. 2003 PMID:14636570

[4] Control of phage Bxb1 excision by a novel recombination directionality factor., Ghosh P. et al, PLoS Biol., 2006. PMID: 16719562.

[5] Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells., Russel JP et al., Biotechniques, 2006. PMID:16629393

[6] A diversity of serine phage integrases mediate site-specific recombination in mammalian cells. Keravala A, Groth AC, Jarrahian S, Thyagarajan B, Hoyt JJ, Kirby PJ, Calos MP. Mol Genet Genomics, 2006 PMID: 16699779