Reporter

Part:BBa_I13605

Designed by: Chris Batton, Victoria Chou, Kenneth Nesmith   Group: Antiquity   (2004-07-16)

Reporter Construct (Ly-Tc-) for constitutive CFP and inducible YFP

Reporter plasmid with Lac operator+YFP(LVA-) reporter followed by a Tet operator+CFP(LVA-) reporter. Inducible with [http://openwetware.org/wiki/IPTG IPTG].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Pictures

[http://openwetware.org/wiki/IPTG IPTG] negative conditions [http://openwetware.org/wiki/IPTG IPTG] positive condition
BBa_I13605 visualized under non-UV light
BBa_I13605 visualized under 254nm UV lightbox
BBa_I13605 grown in IPTG visualized under 254nm UV lightbox
[edit]
Categories
//classic/reporter/multiple
Parameters
emissionCFP, YFP
excitation
tagNone