Composite

Part:BBa_I13540:Design

Designed by: Josh Michener   Group: iGEM04_MIT   (2005-08-22)

Intermediate in two promoter screening plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1880
    Illegal SapI site found at 961


Design Notes

designed for use in CW2553/pJat8 Hogg Keasling


Source

References

<biblio>

  1. Hogg pmid=2656640
  2. Keasling pmid=11739756

</biblio>