DNA

Part:BBa_I13450

Designed by: jkm   Group: iGEM04_MIT   (2005-05-25)


Removes BioBrick End

Contains both an early MfeI site and a late NsiI site. Cutting with Mfe and Spe and ligation to a BB part cut EX will form two mixed sites, neither of which can be recut. Cutting with Xba and Nsi and ligation to a BB part cut SP will again form two mixed sites, neither of which can be recut.


Usage and Biology

Cut MfeI/SpeI in buffer 4 + BSA Cut NsiI/XbaI in buffer 2 + BSA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//dna/cloningsite
Parameters
None