Regulatory

Part:BBa_I12046:Design

Designed by: ryhsiao   Group: Antiquity   (2004-08-23)


Activator for BL oscillator with reporter protein, (cooperativity and L-strain -10 region)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part has cooperativity for the repressor 434 cI (two 434 OR sites). Also, there is a possibility that this Lambda cI promoter region may be more powerful than that of BBa_I12044 since the -10 region has been replaced with that of LLac. A reporter protein (EYFP) coding region is placed after the lambda cI coding region. This will make it possible to keep track of the lambda cI levels in the cell.



Source

References