Part:BBa_I12020:Design
Test Construct of BBa_I12006 promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1227
Illegal BglII site found at 2147 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The test construct was built using BioBrick parts that were available and working. The premise behind this test construct is that the absence of p22 cII will yield a constant promotion of TetR, which will inhibit production of lambda cI. However, upon addition of ATC, the ATC binds to the TetR, thus allowing lambda cI to be promoted. The lambda cI will then bind to the OR-1 and OR-2 regions of BBa_I12006 and thus, promoting the production of Yellow Flourescence Protein. Similarly, the absence of p22 cII will promote the production of LacI in a different subsystem. The LacI will normally inhibit the 434 cI repressor. However, once IPTG is added, the IPTG will bind to the LacI, hence freeing the promoters of the QPI (Quad Part Inverter) and promoting production of 434 cI repressor. The 434 cI repressor will then bind to the O-R3 region of BBa_I12006, hence, inhibiting production of Yellow Flourescence Protein, and thus, turning the switch off.