Coding

Part:BBa_I10343:Design

Designed by: Danielle Carpenter   Group: BE.109 2006 BE.109 Instructors   (2006-04-14)


EGF protein generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 997
    Illegal BamHI site found at 1568
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 263
    Illegal BsaI.rc site found at 1488
    Illegal BsaI.rc site found at 3486


Design Notes

1 EcoRI and 3 PstI sites were removed using <A href="http://slam.bs.jhmi.edu/gd/">GeneDesign</A>. This resulted in a new sequence containing 4868 identites, 9 changes, and 99.8154603239697% identical to the original sequence.


Source

Sequence from NCBI Protein Databank: <A href="http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&val=4503491">NM_001963</A>

References

  • Alemdaroglu C, Degim Z, et al. An investigation on burn wound healing in rats with chitosan gel formulation containing epidermal growth factor. Burns. 2006 May;32(3):319-27 <A href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16527411&query_hl=1&itool=pubmed_DocSum">PMID: 16527411</A>
  • Smith KD, Wells A, Lauffenburger DA. Multiple signaling pathways mediate compaction of collagen matrices by EGF-stimulated fibroblasts. Exp Cell Res. 2006 Apr 2 PMID: <A href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16595133&query_hl=1&itool=pubmed_DocSum">16595133</A>
  • Lee AR. Enhancing dermal matrix regeneration and biomechanical properties of 2nd degree-burn wounds by EGF-impregnated collagen sponge dressing. Arch Pharm Res. 2005 Nov;28(11):1311-6. PMID: <A href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16350860&query_hl=1&itool=pubmed_DocSum">16350860</A>