Part:BBa_I1030:Design
RNA Regulation Target ( Promoter driven by C0040 and cI mod #1 CDS)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 82
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
BBa_I1030 Custom cI Protein with TetR Promoter is based on BioBricks BBa_C0050 and BBa_R0040. It is designed such that BBa_I1031 and BBa_I1032can be used to interfere with the BBa_1030 transcript by anti-sense mRNA binding, in the KISS or micRNA method shown below. BBa_I1030 and BBa_I1031 or BBa_I1032 can act indepently of related biobrick parts BBa_I1041, BBa_I1042, BBa_I1020, BBa_BBa_I1023 and BBa_1013. [<A href="http://biobricks.ai.mit.edu/BB_References.htm#KEIL96">KEIL96</A>]
Incompatible with systems containing IPTG, or cI.
May be some cross talk with systems containing BBa_I1010. Compatible with all other BBa_line I-line parts.
Proper operation requires AMP/CAP complex, which should normally be present in the cell.
Source
see references