Primer
Prefix-R

Part:BBa_G1003:Design

Designed by: Reshma Shetty   Group: iGEM07_Example   (2008-10-17)


Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 16
    Illegal XbaI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 16
    Illegal NotI site found at 8
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 16
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 16
    Illegal XbaI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 16
    Illegal XbaI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This primer sequence is 1 nucleotide shorter than BBa_G1001.


Source

The BioBrick prefix.

References