Primer
Prefix-R

Part:BBa_G1001:Design

Designed by: Jason Kelly   Group: iGEM06_MIT   (2008-04-22)


Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 17
    Illegal XbaI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 17
    Illegal NotI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 17
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 17
    Illegal XbaI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 17
    Illegal XbaI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References