Reporter

Part:BBa_E1011:Design

Designed by: drew (c/o Gustavo Pesce)   Group: Antiquity   (2004-09-03)


mRFP-1 (codon optimized for yeast)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal BamHI site found at 15
    Illegal XhoI site found at 700
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is not in standard alpha version of a biobrick. I.e., there is not a double stop codon, barcode, or bracketing biobrick sites. See subsequent note (email).

Drew- Find below the sequence of the dna I have. It contains yeast optimized mRFP. The aminoacid sequence sequence is taken from Campbel et al. (Tsien). The ORF is flanked by restriction sites of my choice. The fragment is inserted in a blue heron vector called pUCminusMCS. I'll give you some dna of this clone,

Gus



Source

References