Reporter
lacZ a

Part:BBa_E0033:Experience

Designed by: Yong-Su Jin (Fighting Darwins)   Group: Antiquity   (2004-01-27)

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Please enter how you used this part and how it worked out.

Applications of BBa_E0033

User Reviews

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RS

  • RS 11:45, 20 July 2007 (EDT): As currently specified, this coding region lacks an in frame stop codon. There should be an additional T before the AATAA (last 5 nucleotides) in order for the double stop codon to be in frame with the rest of the protein. Sequencing of BBa_E0433 indicates that the sequence in the Registry does reflect the physical part sequence. So the physical part also lacks an in frame stop codon. This may render lacZalpha nonfunctional.

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