Coding
zif23

Part:BBa_C2000:Design

Designed by: Ronny Krashinsky   Group: Antiquity   (2004-01-25)

Repressor, Zif23-GCN4 (RBS-, LVA+)


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As in [Wolfe00], the protein uses residues 364-412 of Zif268: [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=BAC30748.1 here]

The corresponding nucleotide sequence was taken from: <a href="http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=AK040925.1">AK040925.1</a>

As in [Wolfe00], the protein uses residues 250-281 of GCN4: <a href="http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=AAA34640.1">AAA34640.1</a>

The corresponding nucleotide sequence was taken from: [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=171581 K02205.1]

Source

Zif268 and GCN4 [Wolfe00]

Wolfe et al., "Structure of a Designed Dimeric Zinc Finger Protein Bound to DNA" [http://pubs.acs.org/cgi-bin/sample.cgi/bichaw/2003/42/i46/html/bi034830b.html link] Biochemistry 42:13401

References

  • [Wolfe00] Wolfe SA, Ramm EI, Pabo CO. Combining structure-based design with phage display to create new Cys2His2 zinc finger dimers. Structure 21 June 2000, 8:739-750. PMID: 10903945.