Coding
aspA

Part:BBa_C0083:Design

Designed by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)   Group: Antiquity   (2004-01-27)


aspartate ammonia-lyase


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 72
    Illegal BglII site found at 1537
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 964
    Illegal AgeI site found at 493
    Illegal AgeI site found at 1345
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1004


Design Notes

The start codon was changed from GTG to ATG to match BioBricks standards.

Five silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. EcoRI sites were eliminated at bases 588 and 657 with C -> T mutations. PstI sites were eliminated at bases 624, 999, and 1125 with G -> A mutations. The mutations were designed to match codons with similar frequencies of usage in E. coli.



Source

Database Entries:

  • UniProt #: [http://www.pir.uniprot.org/cgi-bin/upEntry?id=ASPA_ECOLI P0AC38;P04422;P78140;Q2M6G5]
  • Entrez Gene #: [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=gene&cmd=Retrieve&dopt=Graphics&list_uids=948658 948658]
  • Source Organism: E. coli K12

References

  • Budrene, E. O. and Berg, H. C. Dynamics of formation of symmetrical patterns by chemotactic bacteria. Nature 376, 49-53 (1995).