Part:BBa_C0078

autoinducer synthetase for PAI from Pseudomonas aeruginosa

coding region for lasI protein, which produces the chemical signal AI-1

Usage and Biology

214 c->t EcoRI restriction site was present. Ttc -> ttt codon (good for E. coli)

402 g->a PstI restriction site was present. Cag -> Caa codon (slightly less optimal for E. Coli)

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 661
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 223
  
• 1000
  COMPATIBLE WITH RFC[1000]

Contribution of SCAU-China 2023

---

We improved this part by optimizing the codons for Escherichia coli expression. Codon optimization was done with the help of GUANGZHOU IGE BIOTECHNOLOGY LTD. We successfully characterized its performance in E. coli TOP10. For more detailed characterization results, please refer to our composite_part-Part:BBa_K4632024[1] and Part:BBa_K4632015[2].