RBS

Part:BBa_B0070

Designed by: Barry Canton   Group: iGEM04_MIT   (2006-03-03)


Specialized RBS modified from that of B0036 (Brink et al.)

This RBS has the modified Shine-Dalgarno sequence, it has a reduced secondary structure relative to using the actual Brink sequence with mixed connective sites flanking it. the spacing between the SD and the ATG is the same as that used by Brink et al. even though it is non-optimal for E. coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rbs/prokaryote/constitutive/miscellaneous
//ribosome/prokaryote/custom
//chassis/prokaryote/ecoli
//direction/forward
//regulation/constitutive
Parameters
None