Help:Cell-free chassis/Characterization

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Several parameters are identified to help us understand the advantages and disadvantages associated with using a particular chassis.

Cell-free chassis specifications (hypothetical graph)
Properties
Definitions
Basic criterion for a chassis Ability to express Biobricks
Temperature dependence The relationship between expression rate and temperature.
Optimum amount of DNA Amount of DNA required for expression rate to reach a maximum value.
Product stability Measure of the half-life of a given protein (e.g. GFP) synthesized in the chassis.
Peak time
Expression lifespan 		Measure of time from start of reaction to the point when expression rate reaches the maximum value
Measure of time from start of reaction to the point when protein degradation overrides protein synthesis
Expression capacity Measure of the total expression of a chassis for a given DNA construct template. This should take into account the degradation of synthesized protein.

To gain knowledge about the cell-free chassis, we have selected the following parts available in the Registry to extract the above-mentioned properties.

  • A simple constitutive gene expression device that requires an E. coli RNA polymerase BBa_I13522
  • A simple constitutive gene expression device that requires a T7 bacteriophage RNA polymerase BBa_I719015
  • An inducible gene expression device that is well-characterized in the registry BBa_T9002