File:Pro-Flipper Chracterisation.jpg
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Results of a highly-efficient one-pot one-step GoldenGate cloning reaction in which a Golden Gate promoter module was inserted into BBa_K1467100 (“Pro Flipper”), replacing the RFP reporter. The BsaI recognition sites have been eliminated by the reaction, converting the part to a standard BioBrick. The very low number of red (RFP-expressing) colonies indicate the extremely high-efficiency of the reaction.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 09:48, 6 October 2014 | | 2,000 × 1,500 (514 KB) | Nicolapatron (Talk | contribs) | Results of a highly-efficient one-pot one-step GoldenGate cloning reaction in which a Golden Gate promoter module was inserted into BBa_K1467100 (“Pro Flipper”), replacing the RFP reporter. The BsaI recognition sites have been eliminated by the reacti |
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