Difference between revisions of "Part:BBa K416001:Experience"
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<partinfo>BBa_K416001 AddReview number</partinfo>
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<partinfo>BBa_K416001 AddReview 3</partinfo>
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<I>Username</I>
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<I>2016 NCTU_Formosa</I>
 
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<p>In the experiment, we hypothesized that an elongated linker would enhance the function of the fusion protein for the original linker is too short for the proper folding of the two domains. Furthermore, we found no result in the previous history using the Flexible Peptide GS Linker. Therefore, we modified the sequence and added three more amino acids from into the linker. In the result, our improved GS Linker is completely proved by our experiments.</p> 
  
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<h3><b>New improved part
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<html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1974030">BBa_K1974030</a></html></b></h3>
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<br>
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<b>Result and Experiment</b>
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In the experiment, the fusion protein with the elongated GS linker did show the enhanced repellent effect. Observed from the phenomenon, we did prove the enhanced function of the fusion proteins, so we speculate that the fusion protein has a better secondary structure than the original ones. As a result, we have submitted a basic part for the improved elongated GS linker.</p>
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[[File:GS_linker_PCR_resullts.png|200px|thumb|center|'''Figure. '''GS linker.
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The DNA sequence length of GS linker is around 50-60 b.p. In this PCR experiment, the product’s size should be close to 300-350 b.p.]]
  
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<b>Uploads</b>
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<b>For more result information on how the function of our GS linker worked in our project, see </b>
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<b><html><a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K1974033">BBa_K1974033</a></html></b>
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Revision as of 10:07, 21 October 2016


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