Difference between revisions of "Part:BBa K3190001"
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The promoter also called GPD promoter regulates the gene for glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH is found in the cytoplasm and the cell wall. It is involved in glycolysis and gluconeogenesis, where it catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate (and the reverse reaction). | The promoter also called GPD promoter regulates the gene for glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH is found in the cytoplasm and the cell wall. It is involved in glycolysis and gluconeogenesis, where it catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate (and the reverse reaction). | ||
− | The TDH3 promoter is used to drive the expression of the TPST1 and TPST2 (Tyrosylprotein sulfotransferase 1 and 2), an enzyme necessary for the relative post-translational modifications of LHCGR receptor. The enzyme catalyzes the sulfation reaction of protein tyrosines. | + | The TDH3 promoter is used to drive the expression of the TPST1 and TPST2 (Tyrosylprotein sulfotransferase 1 and 2), an enzyme necessary for the relative post-translational modifications of LHCGR receptor. The enzyme catalyzes the sulfation reaction of protein tyrosines. Hello |
https://2019.igem.org/wiki/images/0/0f/T--UCopenhagen--Hormones2.png | https://2019.igem.org/wiki/images/0/0f/T--UCopenhagen--Hormones2.png |
Revision as of 08:40, 14 October 2019
pTDH3: Yeast strong constitutive promoter
The promoter also called GPD promoter regulates the gene for glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDH is found in the cytoplasm and the cell wall. It is involved in glycolysis and gluconeogenesis, where it catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate (and the reverse reaction). The TDH3 promoter is used to drive the expression of the TPST1 and TPST2 (Tyrosylprotein sulfotransferase 1 and 2), an enzyme necessary for the relative post-translational modifications of LHCGR receptor. The enzyme catalyzes the sulfation reaction of protein tyrosines. Hello
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]