Difference between revisions of "Part:BBa K1486002:Design"

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===Design Notes===
 
===Design Notes===
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We had to test both possibilities of attaching the superfolded GFP to CpxR, N and C terminus of the CpxR. The C terminus GFP also worked and is submitted as BBa_K1486005. The liker between the two parts was designed for them and can also be found in the registry's catalog.
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We had to test both possibilities of attaching the superfolder GFP to CpxR, N and C terminus of the CpxR. The C terminus sfGFP also worked and is submitted as BBa_K1486005. The liker between the two parts was designed for them and can also be found in the registry's catalog.
  
 
===Source===
 
===Source===

Revision as of 14:50, 25 August 2014

Arabinose promoter + sfGFP CpxR (Nterm)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 2027
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2438
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 1235


Design Notes

We had to test both possibilities of attaching the superfolder GFP to CpxR, N and C terminus of the CpxR. The C terminus sfGFP also worked and is submitted as BBa_K1486005. The liker between the two parts was designed for them and can also be found in the registry's catalog.

Source

The arabinose promoter and the sfGFP were obtained from the registry, while the CpxR was obtained from the E coli genome.

References