Difference between revisions of "Part:BBa K1404002:Design"

 
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<partinfo>BBa_K1404002 short</partinfo>
 
<partinfo>BBa_K1404002 short</partinfo>
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===Design Notes===
 
===Design Notes===
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<p> This parts associates the strong constitutive promoter Ptac (as found in the plasmid pKK233-2, Clontech), a <a  href="http://2009.igem.org/Team:Paris/Parts_RBS"> strong RBS</a> desgined by team Paris Bettencourt 2009, a nickel and cobalt transporter originating from the locus Saro_0344 of <a  href="http://www.ncbi.nlm.nih.gov/nuccore/NC_007794"> <i> Novosphingobium aromaticivorans </i> DSM 12444</a> which was codon-optimised for expression in <i>E. coli</i> and the bidirectional terminator <a  href="https://parts.igem.org/Part:BBa_B0014"> B0014 </a> </p>
  
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===Source===
 
===Source===

Revision as of 10:10, 23 September 2014

Ptac-NiCoTB, improves nickel and cobalt internalization


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 857
    Illegal AgeI site found at 1056
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This parts associates the strong constitutive promoter Ptac (as found in the plasmid pKK233-2, Clontech), a strong RBS desgined by team Paris Bettencourt 2009, a nickel and cobalt transporter originating from the locus Saro_0344 of Novosphingobium aromaticivorans DSM 12444 which was codon-optimised for expression in E. coli and the bidirectional terminator B0014

Source

The promoter and the rest...

References