Difference between revisions of "Part:BBa J100172"
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The M6"-R4 riboswitch is based on the M6" riboswitch, with a strong RBS (AGGAGAA) and sequence that is designed to completely sequester the Shine-Dalgarno sequence when ammeline is absent (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). | The M6"-R4 riboswitch is based on the M6" riboswitch, with a strong RBS (AGGAGAA) and sequence that is designed to completely sequester the Shine-Dalgarno sequence when ammeline is absent (see Figure 1A of http://www.pnas.org/content/107/7/2830.full). | ||
| − | Mutation at position | + | Mutation at position 432 nt of A to G changes tyrosine (Y) to cysteine (C) in RFP. |
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Latest revision as of 18:13, 18 July 2014
Strong Promoter + M6"-R4 Ammeline Riboswitch + RFP
This construct is a generic biosensor for ammeline, based on part J119335. The P5 promoter drives the M6"-R4 riboswitch, which permits translation of the reporter gene (RFP) in the presence of ammeline.
The M6"-R4 riboswitch is based on the M6" riboswitch, with a strong RBS (AGGAGAA) and sequence that is designed to completely sequester the Shine-Dalgarno sequence when ammeline is absent (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).
Mutation at position 432 nt of A to G changes tyrosine (Y) to cysteine (C) in RFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 742
Illegal AgeI site found at 854 - 1000COMPATIBLE WITH RFC[1000]