Difference between revisions of "Part:BBa K4383001"

Revision as of 09:38, 13 October 2022

EryB (erythritol phosphate dehydrogenase)

Erythritol phosphate dehydrogenase in Brucella abortus

the heterogeneous expression of erythritol utilizing pathway could function successfully in E. coli.

figure1.Ery Metabolism pathway

figure2.Ery growth conditions of E. coliDH5a with EryABC, EryRHI, or Ery ABCRHI in M9 erythritol solo carbon source

figure4.Ery growth conditions of E. coliDH5a with pEryU in M9 erythritol solo carbon source

figure4.growth curve of E. coliDH5a in M9 erythritol solo carbon source

figure5.growth curve of pEryU-EcN under the solo carbon source erythritol

figure6.growth curve of pEryU-EcN under a gradient of erythritol, from 0.4g/L to 4g/L in M9 glucose culture

figure7.growth curve of pEryU-EcN under a gradient of different dietary intake proportions of erythritol and glucose

figure8.growth curve of pEryU-EcN,10g/L glucose concentration as the intestinal environment under the normal diet for simulation. 2g/L erythritol to simulate taking 1-2 bottles of erythritol drinking with the normal diet and used 5g/L erythritol + 5g/L glucose, 10g/L erythritol simulating half of the sugar intake was replaced by erythritol, all of the sugar intake was replaced by erythritol, respectively

figure9.RT-qpcr result of pEryU_pUC57-pJ23106-tmd_E. coli

In general, we successfully cloned and characterized the gene for erythritol utilization. The erythritol metabolic pathway was reconstructed in Escherichia coli to make it capable of using erythritol as an independent carbon source for growth. At the same time, we also investigated the effects of different carbon source ratios and different erythritol concentrations on EcN strains that could use erythritol (pEryU-EcN). Finally, we simulated the normal diet and found that a certain amount of erythritol in the normal diet increased the growth of pEryU-EcN, providing validation of our design.

Sequence and Features