Difference between revisions of "Part:BBa K584028"

 
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__NOTOC__
 
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<partinfo>BBa_K584028 short</partinfo>
 
<partinfo>BBa_K584028 short</partinfo>
  
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The pLac-Lux hybrid promoter is repressed by the lacI repressor and induced by IPTG. It is repressed by the luxR repressor bound to the corepressor CO6HSL. It contains a lux box and a lacI binding site. The "Design Notes" portion of this page contains a truth table for this logic gate.  
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<p align="justify">The pLac-Lux hybrid promoter is repressed by the lacI repressor and induced by IPTG. It is repressed by the luxR repressor bound to the corepressor CO6HSL. It contains a lux box and a lacI binding site. The "Design Notes" portion of this page contains a truth table for this logic gate. </p>
  
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The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. '''inaZ''' is the gene responsible for producing the ice nucleation protein.
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<p align="justify">The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. '''inaZ''' is the gene responsible for producing the ice nucleation protein.</p>
  
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We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene '''inaZ'''.
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<p align="justify">We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene '''inaZ'''.</p>
  
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To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the '''inaZ'''.
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<p align="justify">To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the '''inaZ'''.</p>
  
  

Revision as of 07:23, 21 September 2011

pLac-Lux hybrid promoter + INP(Ice Nucleating Protein Generator)

The pLac-Lux hybrid promoter is repressed by the lacI repressor and induced by IPTG. It is repressed by the luxR repressor bound to the corepressor CO6HSL. It contains a lux box and a lacI binding site. The "Design Notes" portion of this page contains a truth table for this logic gate.

The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. inaZ is the gene responsible for producing the ice nucleation protein.

We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene inaZ.

To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the inaZ.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 746
    Illegal NgoMIV site found at 2258
    Illegal NgoMIV site found at 2402
    Illegal NgoMIV site found at 2666
    Illegal NgoMIV site found at 2810
    Illegal NgoMIV site found at 2858
    Illegal NgoMIV site found at 2978
    Illegal AgeI site found at 517
    Illegal AgeI site found at 1922
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 692
    Illegal BsaI.rc site found at 3438
    Illegal SapI.rc site found at 338