Regulatory

Part:BBa_R0182

Designed by: Barry Canton   Group: iGEM04_MIT   (2005-09-26)


T7 RNAP promoter


T7 promoter with an A->T mutation at the -10 position of the consensus sequence


Usage and Biology

This promoter has a single base mutation from the T7 consensus promoter sequence. The mutation is at the -10 base from A to T. The relative strength of this promoter compared to R0085 (the T7 consensus promoter) was measured as 0.3 by Imburgio and coworkers.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//rnap/bacteriophage/T7
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/constitutive
//classic/regulatory/uncategorized
//chassis/bacteriophage/T7
Parameters
negative_regulators
positive_regulators