DNA

Part:BBa_K886000

Designed by: edgar andres ochoa cruz   Group: iGEM12_USP-UNESP-Brazil   (2012-09-24)


Fixed lox71

The particularity of lox71 is that it has an altered sequence at the end of it's right arm compared to loxP. This sequence variation reduces affinity of the Cre recombinase for the arm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//function/recombination/cre
Parameters
chassisE. coli
functionRecombination site recognized by Cre recombinase enzyme