Part:BBa_K4828018
pCMV-NCre
This is based on a part (BBa_K4419025) created by 2022 igem Tokyo Tech, in which the promoter was replaced from the CAG promoter to the CMV promoter and kanamycin and neomycin resistance genes were also incorporated.
fig.1 pCMV-N-Cre
Transformation of this plasmid shows different behavior in the presence or absence of C-Cre (BBa_K4419010): in the absence of C-Cre, EGFP is expressed, resulting in green fluorescence; in the presence of C-Cre, mCherry is expressed, resulting in red fluorescence, as the mature Cre enzyme causes genetic recombination. fluorescence.
Performance Evaluation
Electrophoresis of this plasmid treated with restriction enzyme AflII showed a band at 6.9 kbp, the full length of this plasmid.
fig2. the result of the electrophoresis
The next picture shows the result of transforming this plasmid, and the green fluorescence indicates that pCMV N-Cre was constructed correctly and transformed properly.
fig3. the cells after transformation
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 863
Illegal PstI site found at 1248
Illegal PstI site found at 3078
Illegal PstI site found at 4266 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 863
Illegal PstI site found at 1248
Illegal PstI site found at 3078
Illegal PstI site found at 4266 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 863
Illegal PstI site found at 1248
Illegal PstI site found at 3078
Illegal PstI site found at 4266 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 863
Illegal PstI site found at 1248
Illegal PstI site found at 3078
Illegal PstI site found at 4266
Illegal NgoMIV site found at 2643
Illegal NgoMIV site found at 3397
Illegal NgoMIV site found at 4717
Illegal NgoMIV site found at 5000 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2608
Illegal SapI.rc site found at 4566
Illegal SapI.rc site found at 4776
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