Reporter

Part:BBa_K4799601

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pAtSRO1_GUS_T-Nos

pAtSRO1 is a promoter. It is added before the reporter gene β-Glucuronidase (gus) and NOS terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 309
    Illegal SpeI site found at 835
    Illegal SpeI site found at 1857
    Illegal SpeI site found at 2184
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 309
    Illegal NheI site found at 3993
    Illegal SpeI site found at 835
    Illegal SpeI site found at 1857
    Illegal SpeI site found at 2184
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 309
    Illegal BglII site found at 1975
    Illegal BamHI site found at 1958
    Illegal XhoI site found at 1100
    Illegal XhoI site found at 1132
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 309
    Illegal SpeI site found at 835
    Illegal SpeI site found at 1857
    Illegal SpeI site found at 2184
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 309
    Illegal SpeI site found at 835
    Illegal SpeI site found at 1857
    Illegal SpeI site found at 2184
    Illegal NgoMIV site found at 2408
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2208
    Illegal BsaI.rc site found at 2823
    Illegal SapI.rc site found at 2994


[edit]
Categories
Parameters
None