Coding

Part:BBa_K3258023

Designed by: Ayush Pandit   Group: iGEM19_Stanford   (2019-10-20)


Dead Frameshift version of AcrIIA4 AntiCRISPR protein

A "dead" nonfunctioning version of AcrIIA4 with the deletion of 2 A amino acids immediately following the start codon, resulting in a 2 frameshift mutation. Used as a negative control with AcrIIA4 experiments.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 244


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Categories
Parameters
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