Composite

Part:BBa_K3206006

Designed by: Matthew Rogan   Group: iGEM19_Newcastle   (2019-10-15)


Constituitively expressed ChaC gene

This composite part consists of a BBa_J23100 promoter which allows constitutive expression of the gene ChaC. ChaC encodes a glutathione-specific gamma-glutamylcyclotransferase, which catalyses the cleavage of glutathione into its main component parts (5-oxo-L-proline and cysteine-glycine dipeptide). This part can be used for glutathione degradation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 196
    Illegal AgeI site found at 263
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
transcriptional
Parameters
biologyEscherichia coli K12
proteinChaC