Coding

Part:BBa_K2029005

Designed by: Max Schelski, Niklas Schmacke   Group: iGEM16_UBonn_HBRS   (2016-10-14)


EngB

Part coding for catalytic enzyme endoglucanase, which cleaves 1,4-beta-D-glycosidic linkages in cellulose. This specific gene sequence is naturally found in some strains of Clostridium cellulovorans.

Usage and Biology

The enzymes expressed can be used to hydrolyse cellulose molecules into simple sugars.

This gene is intended for use in B. subtilis, preceded by a secretory tag like BBa_K2029000 or BBa_K2029001 which signals the B. subtilis cell to secrete the coded protein into the medium.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None